Journal: Stem Cells International

Article Title: Mesenchymal Stem Cells Combined with Tissue Fusion Technology Promoted Wound Healing in Porcine Bowel Anastomosis

doi: 10.1155/2020/5142797

Figure Lengend Snippet: MSC tracking and differentiation. (a) Tracking of grafted MSCs (50x) on d 14. (b) Tracking of grafted MSCs (400x) on d 14, showing a zoomed-in view of (a). (c) Differentiation of smooth muscle cells from grafted MSCs (50x) on d 14. (d) Differentiation of smooth muscle cells from grafted MSCs (400x) on d 14, showing a zoomed-in view of (c). Red: CM-Dil fluorescent dye. Green: anti-alpha smooth muscle actin in (a, b) and pan cytokeratin in (c, d). Blue: 4,6-diamidino-2-phenylindole.

Article Snippet: To label cultured ADMSCs with chemical dye for later tracking, 1 × 10 6 DMSCs were labeled with 5 μ L of CM-Dil fluorescent dye (Invitrogen) for 20 min in an incubator at 37°C and then washed with PBS three times.

Techniques:

Journal: Membranes

Article Title: CM-Dil Staining and SEC of Plasma as an Approach to Increase Sensitivity of Extracellular Nanovesicles Quantification by Bead-Assisted Flow Cytometry

doi: 10.3390/membranes11070526

Figure Lengend Snippet: Efficacy of plasma component labeling with CM-Dil. ( A ) Plasma (2 mL) and ENVs isolated from 2 mL plasma were stained with CM-Dil (1000 pM), SEC fractioned, and assayed be means of flu-dot-blot analysis. The results were quantified using an Invitrogen iBright FL1500 Imaging System. ( B ) Plasma samples (2 mL) were stained with CM-Dil at concentrations of 1000 pM, 50 pM, and 2.5 pM; SEC fractionated; and assayed by means of fluo-dot-blot analysis. The results were quantified using an Invitrogen iBright FL1500 Imaging System.

Article Snippet: The fluorescence intensity of the CM-Dil dye was measured on an Invitrogen iBright FL1500 Imaging System with an exposure time of 0.016 s. The values were digitized using the ImageJ program.

Techniques: Labeling, Isolation, Staining, Dot Blot, Imaging

Journal: Membranes

Article Title: CM-Dil Staining and SEC of Plasma as an Approach to Increase Sensitivity of Extracellular Nanovesicles Quantification by Bead-Assisted Flow Cytometry

doi: 10.3390/membranes11070526

Figure Lengend Snippet: Dot-blot analysis of SEC fractions of plasma and CM-Dil-stained plasma. Particle suspensions obtained via fractionation were spotted, stained with antibodies, and their signal intensity was evaluated with HRP-labeled secondary antibodies and HRP substrate. ( A ) Dot-blot images after CD63 analysis; ( B ) results of all data quantifications with the Invitrogen iBright FL1500 Imaging System.

Article Snippet: The fluorescence intensity of the CM-Dil dye was measured on an Invitrogen iBright FL1500 Imaging System with an exposure time of 0.016 s. The values were digitized using the ImageJ program.

Techniques: Dot Blot, Staining, Fractionation, Labeling, Imaging